The Challenges with Traditional TFF
Tangential flow filtration (TFF) relies on recirculation to control membrane fouling, but most TFF systems were designed for large volumes. As R&D shifts toward smaller, higher-value batches, traditional systems become inefficient, wasteful, and difficult to automate.
- Minimum Volume Mismatch — Existing systems require 5–10 mL minimum working volumes, wasting material when only sub-mL samples are needed.
- Manual Operation — Most benchtop systems require manual control and close monitoring when processing low-volume samples, making automation impractical.
- Scale-Up Discontinuity — R&D may be forced to use alternative purification methods, and the results often don't transfer to production, requiring additional process development at larger scales.
Our First-Principles Design Approach
Tanda systems are designed from first principles to eliminate unnecessary volume, complexity, and operator dependence.
- Minimized Hold-Up Volume — Absolute minimum tubing paths reduce sample loss and batch size.
- Small Format Filters — Small hollow fiber filters with a minimum filtration surface area of 4 cm² or less reduce tubing length and hold-up volume.
- Passive Pressure Control — Application-driven flow path design eliminates the need for pressure sensors and back-pressure valves.
- Automated Process Termination — Weight-based permeate monitoring stops the process automatically — no manual intervention required.
The Results
Lower cost： Routine processing of sub-mL samples preserves valuable or hazardous materials.
Higher throughput： Minimal training, fast setup, and small footprint enable parallel processing.
Straightforward tech transfer： Consistent filter formats and well-understood laminar flow simplify scale-up.
How We Compare
| Capability | Tanda Systems | Others |
|---|---|---|
| Minimum Working Volume | <0.5 mL | 5–10 mL |
| Hold-Up Volume | <0.5 mL | 5–15 mL typical |
| Pressure Control | Passive pressure control | Pinch valve with manual or P&ID |
| Setup Time | 5 minutes (ReadyPath™) | 30–60 minutes |
| Automation Level | Walk-away operation | Manual or automated |
| Scalability | Directly scalable filter design | May need different filter technologies for sub-mL sample volumes |
| Alternative to Dialysis | Faster and easier alternative to dialysis | Faster but much more complicated than dialysis |
Selected Applications
- LNP & RNA Therapeutics — Buffer exchange and concentration for mRNA and siRNA formulations. Low hold-up volume is critical when working with expensive nucleic acid payloads.
- Exosomes & EVs — Gentle isolation and purification at research-relevant volumes. Our low-shear flow paths preserve vesicle integrity during processing.
- Proteins & Biologics — Concentration, diafiltration, and formulation. TxFlow™ Go handles 0.5–500 mL; custom systems scale to production volumes.
- Nanoparticles — Purification and concentration of nanoparticles after reaction.
- Faster Alternative to Dialysis — Complete buffer exchange in 30 minutes to a few hours instead of days, while reducing buffer consumption by >95% through automated diafiltration.
Questions about your application? Talk to an engineer or email support@mtwbiotech.com